Effect of transforming growth factor ? on proliferation of prostatic cells.
- Author:
Hyun Joo KIM
1
;
Jae Dam LEE
;
Han Jong AHN
Author Information
1. Department of Urology, College of Medicine, University of Ulsan, Asan Medical center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
TGF-beta1;
Tissue culture;
Prostate
- MeSH:
Cell Proliferation;
Cholera Toxin;
Collagen;
Digestion;
Epidermal Growth Factor;
Epithelial Cells;
Extracellular Matrix;
Fibroblasts;
Fluorescent Antibody Technique;
Fluorescent Antibody Technique, Indirect;
Humans;
Hydrocortisone;
Insulin;
Keratins;
Prostate;
Prostate-Specific Antigen;
Selenium;
Transforming Growth Factor beta1;
Transforming Growth Factors*;
Vimentin
- From:Korean Journal of Urology
1993;34(5):770-777
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Transforming-growth factor beta l, a prototypital autocrine growth factor, exhibits such a remarkable diversity of biological activity that may stimulate or inhibit cellular proliferation and stimulate extracellular matrix formation depending on the cell type. We evaluated the effect of TGF-beta1 on proliferation of the human prostatic epithelial cell and fibroblast. Epithelial cells were released from tissues by collagen digestion, and primary and subcultures cells were grown in PFMR-4A medium supplemented with epidermal growth factor, phosphoelhanolamine, cholera toxin, selenium, insulin, hydrocortisone, and bovine pituitary extract. Prostate-derived fibroblasts were cultured using RPMI 1640 medium with 10% fetal bovine serum. Verification of prostatic epithelial cells was accomplished by indirect immunofluorescence detection of prostate specific antigen, cytokeratin, and prostate-derived fibroblast was confirmed by immunofluorescence detection Of vimentin. Proliferation assay was performed using 3H-thymidine assay. Time and dose dependent inhibitory effects of TGF-beta1 on proliferation of prostatic epithelial cells were noted (86+/-6% at 0.1ng,ml and 21+/-3% at 10ng/ml after 96hr exposure). while TGF-beta1 at a high concentration stimulated the proliferation of prostate-derived tibroblasts (125+/-11% at lng/ml after 48hr exposure, 118+/-10% at 1ng/ ml arter 96hr exposure). However in the absence of fetal bovine serum, TGF-betal, at a physiologic range of 0.01 ng/ml showed inhibitory effect on proliferation of prostate-derived fibroblasts. These data show that TGF-beta1, a growth modulating cytokine, regulates growth of prostatic epithelial cells and prostate-derived fibroblast in the different way.
