Determination of related substances of vitamin B6 for injection by HPLC
- VernacularTitle:高效液相色谱法测定注射用维生素B6有关物质
- Author:
Yu ZHAI
;
Fang YANG
;
Weiqun ZHANG
- Publication Type:Journal Article
- Keywords:
Vitamin B6;
High performance liquid chromatography
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(2):281-282
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method to determine the related substances of vitamin B6 for injection.Methods HPLC method was adopted with DiamonsilTM C18,5μm 250mm×4.6mm;the mobile phrase was length was 291nm.Results The calibration curve was linear in the concentration range of 600～1400μg/ml(r=0.9998).Conclusion The method is simple,rapid,accurate and reliable.As the supplement method of active pharmacopoeia,it can effectively control the quality of the product.
