Prevalence of Ambler Class A Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea.
- Author:
Ji Hae KANG
1
;
Il Kwon BAE
;
Su Bong KWON
;
Seok Hoon JEONG
;
Jongwook LEE
;
Wee Gyo LEE
;
Jung Oak KANG
;
Ji Young AHN
;
Seong Geun HONG
;
Jong Hee SHIN
;
Young UH
;
Yeon Jun PARK
;
Eui Chong KIM
;
Kyungwon LEE
;
Dongeun YONG
;
Gun Jo WOO
Author Information
1. Department of Pediatrics, Kosin University College of Medicine, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Extended-spectrum beta-lactamase;
CTX-M;
GES-3;
E.coli;
K.pneumoniae
- MeSH:
Agar;
Anti-Bacterial Agents;
Base Sequence;
beta-Lactamases;
Ceftazidime;
Diffusion;
Escherichia coli*;
Escherichia*;
Genotype;
Klebsiella pneumoniae*;
Klebsiella*;
Korea*;
Polymerase Chain Reaction;
Prevalence*
- From:Korean Journal of Clinical Microbiology
2005;8(1):17-25
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The aim of this study is to determine the nationwide prevalence of Ambler class A extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae and to characterize genotypes of ESBLs. METHODS: During the period of February through July, 2003, E.coli and K.pneumoniae isolates were collected from 12 hospitals in Korea. Antimicrobial susceptibilities were tested by disk diffusion method, and ESBL-production was determined by the double-disk synergy test. MICs of beta-lactam antibiotics were tested by agar dilution method. Searches for bla TEM, bla SHV, bla CTX-M, bla PER-1, bla VEB, bla IBC, bla GES and bla TLA genes were performed by PCR amplification, and the genotypes of ESBLs were determined by direct nucleotide sequence analysis of amplified products. RESULTS: Resistance rates of E.coli (n=246) and K.pneumoniae (n=239) isolates to ceftazidime were 8.5% and 20.1%, respectively. Most prevalent Ambler class A ESBL genotypes in E.coli isolates were bla CTX-M-15 (n=4) and bla CTX-M-3 (n=3), and each of bla CTX-M-14, bla SHV-12, and bla TEM-52 gene was also found in one isolate. Most prevalent ESBL genotypes in K.pneumoniae were bla SHV-12 (n=30) and bla CTX-M-3 (n=13), and bla CTX-M-14 (n=5). bla SHV-2a (n=3), bla SHV-5 (n=2), bla TEM-52 (n=1), bla GES-3 (n=2) genes were also found. CONCLUSION: CTX-M-type ESBL-producing E.coli and K.pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.
