Expression changes of microRNA array in mesencephalon of rats with traumatic coma
- VernacularTitle:脑损伤昏迷大鼠中脑神经组织microRNA表达谱的变化特征
- Author:
Guoyi GAO
;
Xian FU
;
Yumin LIANG
;
Yinghui BAO
;
Junfeng FENG
;
Kuiming ZHANG
;
Lei Lü
;
Jiyao JIANG
- Publication Type:Journal Article
- Keywords:
Brain injuries;
Coma;
Mesencephalon;
Rats;
Gene expressin chip
- From:
Chinese Journal of Trauma
2008;24(4):274-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a rat model with acute traumatic coma and identify the variation of microRNA in mesencephalon. Methods After rats were injured moderately by central fluid percussion system, tissues of the mesencephalon were removed one hour after injury. RNA of brain tissue of the mesencephalon was isolated for microRNA array by using the exiqon microarray system. The data were analyzed statistically by Genepix Pro 6.0 after hybridization results were scanned and fluorescence intensity standardized. Resets Hybridization results showed 33 microRNAs with up-regulated expressions but 38 microRNAs with down-regulated activity. Conclusion Expression of microRNA array shows marked changes in the tissues of the mesencephalon in rats with traumatic coma, as may be injury mechanism of traumatic coma and also a way of neurobiological protection of coma.
