Effects of keto-valine-calcium and keto-isoleucine-calcium on proliferation of human mesangial cells and its mechanism study
- VernacularTitle:酮缬氨酸钙与酮异亮氨酸钙对人肾系膜细胞增殖影响及机制研究
- Author:
Jie LI
;
Ping ZHOU
;
Xiumei HU
;
Fuyou LIU
;
Youmin PENG
- Publication Type:Journal Article
- Keywords:
Valine;
Leucine;
Glomerular mesangium;
Cell proliferation
- From:
Journal of Chinese Physician
2008;10(4):489-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of keto-valine-calcium and keto-isoleucine-calcium on(human mesangial cells)HMCs. Method HMCs were stimulated by keto-valine-calcium and keto-isoleucine-calcium. The AT1R and TGF-β1 were detected. MTr method was used to measure the proliferation of HMCs, and cell cycle was studied by flow cytometry. Results The expression of AT1R and TGF-β1was increased in the experiment groups compared with negative and DMSO control groups. Cell cycle G1 attest and cell apoptosis were observed in the experiment groups. Conclusions 10mM keto-valine-calcium and keto-isoleueine-calcium have multiple effects on HMCs in vitro, which not only increased the expression of AT1R,but also the expression of TGF-β1.Furthermore,keto-valine-calcium and keto-isoleucine-calcium can induce cell cycle G1 arrest and cell apoptosis.
