SonoVue and ultrasound mediated pEGFP-N1 transfection to mouse cornea in vivo study
- VernacularTitle:超声微泡造影剂介导pEGFP-N1转染小鼠角膜的实验研究
- Author:
Ying WU
;
Lianfang DU
;
Yongdong CHEN
;
Huiping WANG
;
Feng WANG
- Publication Type:Journal Article
- Keywords:
Ultrasonograplly;
Gene transfer tedhniques;
Cornea
- From:
Chinese Journal of Ultrasonography
2008;17(4):350-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficiency and safety of transfection green fluorescent protein plasmid to mouse cornea mediated by SonoVue and ultrasound.Methods Saline,plasmid+saline,plasmid+SonoVue and liposome+plasmid were injected respectively to mouse eye anterior chamber.Then the mouse eye of plasmid+saline and plasmid+SonoVue injection group were exposed to pulse wave ultrasound under 50 Hz pulse reDetition frequence,2 W/cm2 intensity and 10 minutes duration time.Fluorescence stereomicroscope was used to observe the expression of EGFP in the eye at the 1 st day,3rd day,7th day,14th and 21 st day after injection.Two mice were taken randomly from each group and were sacrificed at the 3rd day after injection.Their eyes were enucleated and made into frozen coronal sections.And fluorescence microscopy was performed to observe the type and distribution of EGFP positive cell.Tissue damage was observed in pathological section.Results EGFP was exDressed over the ocular surface in SonoVue and ultrasound group,and it obviously higher than only ultrasound exDosure group and liposome group.The expression of EGFP was not detect ed over the ocular surface in other groups.Pathological sections were not found any difference in each group.Conclusions SonoVue and ultrasound can successfully and safely transfer gene to ocular in vivo study.
