The influence of puerarin on the expression of TNF-α in rats with focal cerebral ischemia and reperfusion
- VernacularTitle:脑缺血再灌注损伤后TNF-α表达的变化及葛根素的干预作用
- Author:
Ping ZHANG
;
Tong LI
;
Bin ZHU
;
Bin YUAN
- Publication Type:Journal Article
- Keywords:
Puerarin/PD;
Brain ischemia;
Reperfusion injury/PC;
Tumor necrosis factor-alpha
- From:
Journal of Chinese Physician
2008;10(4):447-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the nerve protective mechanism of puerarin on ischemic brain injured and observe the expression change of TNF-α cytokines in ischemic brain area. Methods 90 healthy male SD(Spraque-Dawley)rats were used to built the animal model of brain medium sized artery focal cerebral ischemia reperfusion with the patching methods, and the rats were randomly divided into three groups: Sham operation group(n=30),operation group(n=30),operation with puerarin interfered group(n=30).There were five different observing time in every group(6h,12h,24h,48h,72h after operation).The operation with puerarin group rats were treated with puerarin solution, the sham operation and operation group rats were injected the same volume Sodium Chloride. To observe the expression of TNF-α cytokines in different time, immunohistochemistry staining in ischemic region was used. Results Compared with the sham operation group. the expression of TNF-α cytokine in cerebral isehemic reperfusion brain tissue in the operation and the operation with puerarin interfered groups was higher(P＜0.01).Compared with the operation group, the expression of TNF-αcytokine was significantly decreased in different time after cerebral ischemic-reperfusion in the operation with puerarin interfered group(P＜0.01 or P＜0.05).Conclusions The puerarin can restrain the expression of the TNF-α cytokine.
