Effects of cyclosporine on cell proliferation, apoptosis and angiogenesis of bladder cancer in rats
- VernacularTitle:环孢素对大鼠膀胱肿瘤细胞增殖、凋亡和新生血管形成的影响
- Author:
Xiaojun ZHAO
;
Liang REN
;
Xiaodong ZHANG
;
Yong WANG
;
Shihai LI
;
Hang YIN
;
Xiaopeng HU
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Bladder neoplasms;
Proliferation;
Apoptosis;
Angiogenesis
- From:
Chinese Journal of Urology
2008;29(5):342-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of cyclosporine on cell proliferation, apoptosis and angiogenesis of bladder cancer in rats induced with BBN.Methods Ten samples of SD rats bladder cancer induced with BBN and cyclosporine simultaneously and 10 samples of SD rats bladder cancer induced with BBN only as control were used to observe the effects of cyclosporine on cell proliferation, apoptosis and angiogenesis of bladder cancer in rats.Immunohistochemistry stain with PCNA, TUNEL and immunohistochemistry stain with CD31 were used to analyze cell proliferation,apoptosis and angiogenesis of bladder cancer in rats respectively. Results The differences of proliferation index (35.3±8.6)% and (28. 7±8.0)% respectively (P＜0.05), apoptotic index (2. 5±0.8)% and (4.3±1.3)% respectively (P＜0.05) and microvessel density 32.5±8.2 and 26.3±8.1 respectively (P＜0.05) between experimental group and control group were all significant.Conclusions Cyclosporine may stimulate the growth and development of bladder cancer through mechanisms of cell proliferation, apoptosis and angiogenesis.
