Antimicrobial susceptibility of Haemophilus influenzae strains isolated from pediatric patients with acute respiratory infection and bacterial meningitis in Hanoi
- Author:
Dang Duc Anh
- Publication Type:Journal Article
- Keywords:
Haemophilus influenzae strain;
Pediatric patient;
Acute respiratory infection;
Bacterial meningitis;
Antibacterial susceptibilit
- MeSH:
Haemophilus influenza;
Pediatrics;
Meningitis;
Bacterial Infections
- From:Journal of Preventive Medicine
2005;15(1):29-32
- CountryViet Nam
- Language:Vietnamese
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Abstract:
The molecular characteristics of 44 H. influenzae strains were isolated from children with bacterial meningitis and 37 strains were isolated from children with acute respiratory infection were investigated. Of the 37 H. influenzae isolates from nasopharyngeal aspirates (NA), the serotype of 30 isolates were nontypeable, 4 were type b, 2 were type c and one was type a. Whereas those of 44 isolates from cerebrospinal fluid (CSF) were all type b. Twenty-six (70.3%) from NA and 23 strains from CSF were beta lactamase-producing, and the remaining strains were beta lactamase negative. PCR analysis to identify the resistant genes indicates 25 strains from NA and 21 strains from CSF had TEM-l-type-beta lactamase gene, 11 strains from NA and 22 strains from CSF lack all resistant genes, and each strain from NA and CSF had TEM-l-type beta lactamase gene and fts I gene with the same substitution as the low -betalactamase-negative ampicillin resistant (low-BLNAR) strain. No BLNAR strain was found. The MICs against 16 antibiotics between isolates from NA and CSF were similar except for penicillin G and ampicillin.
