The effects of TCM YiShen decoction on the expression of PAI-1 in kidney tissue of mice with IgA nephropathy
- VernacularTitle:PAI-1在IgA肾病小鼠肾组织中表达的变化及益肾汤对其的影响
- Author:
Qijun WAN
;
Zhengzhi WU
;
Yongcheng HE
;
Chengang SHI
;
Guobao HONG
;
Bin HU
;
Shaodong LUAN
- Publication Type:Journal Article
- Keywords:
Plasminogen activator inhibitor 1;
Glomerulonephritis,IgA;
Neoplasms;
Kidney-reinforcing drugs/PD;
Decoctions
- From:
Journal of Chinese Physician
2008;10(4):502-504
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore mRNA and protein expression of PAl-1 in kidney tissue of mice with IgA nephropathy (IgAN) and the effects of YiShen Decoction. Methods The IgAN model was built by the method of oral intake of bovine serum albumin(BSA)together with the injection of staphylococcus enterotoxin B(SEB)through caudal vein. The mRNA expression of PAI-1 was measured by fluorescent quantitative polymerase chain reaction (FQ-PER)and the expression of PAI-1 protein was measured by immunohistochemistry. Results No significant difference of mRNA and protein expression of PAI-1 was found between the low concentration and high concentration Yishen Decoction group. But the secretion and mRNA expression of PAI-1 in the low and hiigh concentration Yishen Decoction group was decreased more than that in the IgAN model group. Conclusion The abnormal expression of PAI-1 mRNA and protein played an important role in the onset and development of IgAN. The TCM Yishen Decoction could reduce the abnormal expression of the mRNA and protein of PAI-1 in kidney tissue of mice with IgA nephropathy.
