Expression of NF-E2 related factor in vitiligo lesions
- VernacularTitle:白癜风患者皮损NF-E2相关因子2的表达
- Author:
Cuiping GUAN
;
Xiaodong WEI
;
Miaoni ZHOU
;
Yongwei LI
;
Dekuang ZHAO
;
Aie XU
- Publication Type:Journal Article
- Keywords:
Vitiligo;
Oxidative stress;
NF-E2-related factor 2
- From:
Chinese Journal of Dermatology
2008;41(5):324-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the level of NF-E2 related factor 2 (Nrf 2) in vitiligo lesions.Methods Tissue samples were obtained by press suction blisters at lesional and donor sites of 12 patients with vitiligo who were managed with epidermal transplantation. Four lesional samples from the patients were subjected to primary culture and the level of Nrf 2 was detected by AEC immunohistochemistry after 48hours of culture. Western blotting was utilized to further detect the level of cytoplasmic and nuclear Nrf 2 in tissue samples from the other 8 patients with vitiligo. Results Immunohistochemistry revealed that Nrf 2 was predominantly expressed in cytoplasm, rather than nuclei, of keratinocytes in vitiligo lesions compared with the normal skin of patients. The level of nuclear Nrf 2 was significantly lower in lesions than that in normal skin (0.10 ± 0.03 vs 0.26 ± 0.03, P ＜ 0.01) of the patients. In contrast, there was no significant dif- ference in the level of cytoplasmic Nrf2 between lesional and normal skin (0.61 ± 0.03 vs 0.60 ～ 0.02, P ＞0.05) of patients. Conclusion These results reveal an abnormality of nuclear translocation of Nrf 2 in vitili-go lesions.
