Inhibiting effect of interleukin-10 on expressions of intercellular adhesion molecule-1 and P-selectin in cerebral ischemia-reperfusion foci of rats
- VernacularTitle:白介素-10对大鼠脑缺血再灌注血管内皮细胞间黏附分子-1和P-选择素的抑制作用
- Author:
Nan LIU
;
Zhihua WU
;
Houwei DU
;
Ronghua CHEN
;
An ZHENG
;
Huapin HUANG
- Publication Type:Journal Article
- Keywords:
Interleukin-10;
Cell adhesion molecules;
Brain ischemia;
P-selectin
- From:
Chinese Journal of Geriatrics
2008;27(5):376-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibiting effect of interleukin-10 on the expressions of intercellular adhesion molecule-1(1CAM-1)and P-selectin in cerebral ischemia and reperfusion foci(I/R)in rats. Methods Sixty adult male Sprague-Dawley rats were assigned to 4 groups randomly:Sham operated group(n=6),cerebral I/R group(6,24,48 hours post I/R,each had 6 rats),vehicle group(6,24,48 hours post I/R,each had 6 rats)and IL-10 treatment group(6,24,48 hours post I/R,each had 6 rats).I/R rat model was established by middle cerebral artery occlusion(MCAO)with Longa's thread method. Immunohistochemical staining and retro-transcriptase polymerase chain reaction were used to detect the expressions of ICAM-1 and P-selectin. Results ICAM-1(31.60±1.85,54.11±2.32,48.61±2.30)and P-selectin(29.39±5.16,50.44±4.38,37.89±4.74)expressions on endothelial cells 6,24 and 48 hours post I/R were significantly upregulated as compared with sham group(7.61±1.14,0,respectively)(alI P＜0.01),and the expressions of ICAM-1 (24.06±2.42,42.00±3.26,45.28±2.91)and P-selectin(27.28±1.84,42.78±2.51,36.00±3.22)on endothelial cells of IL-10 treatment group 6h,24h,48 h post I/R were significantly suppressed at any timepoint in comparison with vehicle group(P＜0.05). Conclusions IL-10 could inhibit the expressions of ICAM-1 and P-selectin on endothelial cells of cerebral I/R foci in rats.
