T lymphocyte subsets and intracellular cytokines after transfer of chemical acellular nerve allograft
- VernacularTitle:去细胞异体神经移植后T细胞亚群及细胞内细胞因子的实验研究
- Author:
Wei LI
;
Hongbin ZHONG
;
Xingshi LIN
;
Shuxun HOU
;
Wenwen WU
;
Dike YUAN
- Publication Type:Journal Article
- Keywords:
Peripheral nerve;
Nerve graft;
Immunity;
T lymphocyte subsets;
Lymphokines
- From:
Chinese Journal of Orthopaedic Trauma
2008;10(5):450-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide immunological evidence for clinical transfer of chemical extracted acellular nerve allografL Methods One hundred and twenty-eight BALB/C mice were randomly divided into 4 groups of equal size according to their different treatments:negative contrast group(NC),fresh autograft group(AG),fresh allogeneic nerve group(FN)and chemical extracted aceflular allogeneic nerve group(CEN).Then we implanted various kinds of nerve grafts into the thigh muscle of BALB/C mice in corresponding groups.At 3,7,14,28 days postoperatively,8 mice from each group were killed each time to harvest their spleens,from which T lymphocytes were collected.Theu monoclonal antibodies(CD3,CD4 CD8 CD25,IL-2,IFN-γ, TNF-α)were added into the suspension.Then fluorescence.activated cell sorting(FACS)was used to determine the positive rates of cells combined with the above monoclonal antibodies. Results There were no statistically significant differences between CEN group,NC group,and AG group,but indexes of FN group were significantly higher than those of the other 3 groups at corresponding time points. Conclusion There is no obvious immune reiection of chemical extracted acellular nerve allograft when compared with fresh nerve autograft.
