The roles of TANK-binding kinase-1 in chronic hepatitis B virus infection induced interferon antiviral immunity
- VernacularTitle:TANK结合激酶1在乙型肝炎病毒诱导抗病毒反应中的表达及作用
- Author:
Baoyan AN
;
Qing XIE
;
Hui WANG
;
Siming GUO
;
Nina JIA
;
Huaicheng SHEN
;
Lanyi LIN
;
Wei CAI
;
Hong YU
;
Qing GUO
- Publication Type:Journal Article
- Keywords:
TANK-binding kinase-1;
Interferon beta;
Dendritic cells;
Hepatitis B virus
- From:
Chinese Journal of Infectious Diseases
2008;26(5):282-286
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the roles of TANK-binding kinase-1(TBKl)in hepatitis B virus (HBV)infection induced interferon antiviral immunity.Methods Peripheral blood monocytes were separated by CD14 magnetic microbeads from healthy volunteers(HV)and chronic hepatitis B(CHB)patients.Purified mDCs were induced and proliferated in the culture medium with human granulocyte-macrophage concentration of 25 mg/L were stimulated.The mRNA expressions of TBK1,interferon regulatory factor (IRF)3 and interferon(IFN)-βwere quantified by real time polymerase chain reaction(PCR).The levels of IFN-β in supernatants were determined by enzyme-linked immunosorbent assay(ELISA).Reslllts The mRNA levels of TBK1,IRF3 and IFN-β did not change significantly at 0,12,24 and 48 h after the significantly at 0, 12, 24 and 48 h in CHB group, whereas, it was significantly up-regulated at 12 h in HV group. Conclusions Our results suggest that there may be some disorders in host antiviral signal transduction pathways downstream the binding between ligands and receptors on mDC surface. The insufficient IFN-β expression after HBV infection may result in persistent chronic infection.
