Effect of donor hepatocyte preinfusion on the survival of islet xenografts
- VernacularTitle:同供体肝细胞预先输入对异种胰岛移植物存活影响的实验研究
- Author:
Shi CHENG
;
Yongfeng LIU
;
Sanguang HE
- Publication Type:Journal Article
- Keywords:
Hepatocytes Preinfusion Islet xenograft Survival
- From:
Chinese Journal of Organ Transplantation
1998;19(4):208-210
- CountryChina
- Language:Chinese
-
Abstract:
The rat islets were isolated by using collagenase digestive method, purified by Fi-coll density gradient centrifugation, and rat hepatocytes isolated simultaneously.Twenty-four cas-es of diabetic models, developed in mice with streptozotocin, were divided into 3 groups randomly.In experimental group, donor rat hepatocytes were infused first, followed by transplantation of cultured islets from same donor via portal vein on the 6th day.In Control group 1 no intervention was performed, and in control group 2, simple infusion of islets was done.The results showed group.Significant difference was noted between the two groups(P＜0.05).The decreased blood glucose level were maintained about a week in experimental group.This result reveals that hepa-tocytes can induce immunotolerance and prolong the survival of xenografts to some extent.
