The effects of granulocyte colony-stimulating factor in reversion of the apoptosis of poly morphonuclear neutrophil in acute lung injury
- VernacularTitle:急性肺损伤炎性细胞凋亡异常及粒细胞集落刺激因子调控的研究
- Author:
Shouxin HAN
;
Fuhui CHEN
;
Dan ZHOU
;
Xiaomei WU
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Granulocyte colony-stimulating factor
- From:
Clinical Medicine of China
2008;24(5):431-432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis of poly morphonuclear neutrophil(PMN)and the reversion of recombinant human granulocyte colony-stimulating factor(G-CSF)in acute lung injury.Methods 30 guinea pigs were randomly divided into three groups:control group,oil acid group(OA group),OA+G-CSF group.Both OA group and OA+G-CSF group had intraveneos injection of oleic acid(0.12ml/kg)to induce acute lung injury.OA+G-CSF group had G-CSF 0.5μg/kg injection once a day for 2 days.Control group had injection of normal saline.All the 3 groups took BALF 2 hours later.PMNs were isolated by density gradient centrifugation.PMN apoptosis was detecded by Terminal deoxynucleotidy transferase-mediated dUTP biotin nick end labeling(TUNEL).Results PMN apoptosis of BALFs of OA group,OA+G-CSF group and control group were(2.5±1.080)%,(3.5±0.850)%and(6.4±1.505)%.The level of PMN apoptosis of BALF of OA group compared with OA+G-CSF group and control group were decreased markedly(P＜0.01 for each).Conclusion The apoptosis of PMN in acute lung injury is delayed,and persistent activation of PMN and release of toxic content is closely related to lung injury.G-CSF can reverse the level of apoptosis of PMN in acute lung injury.
