Apoptosis of peripheral blood lymphocytes activated by allospecific antigen
- VernacularTitle:人外周血淋巴细胞Fas的表达及细胞凋亡的诱导
- Author:
Liangli WANG
;
Ping ZOU
;
Zhongbo HU
- Publication Type:Journal Article
- Keywords:
Lymphocytes;
Antigens;
Gene exprexsion;
Apoptosis
- From:
Chinese Journal of Organ Transplantation
2000;21(3):156-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to study the effect of the Fas system on normal peripheral blood lymphocytes(PBL)activated by allospecific antigen.Methods The isolated and purified lymphocytes werc subjected to one-way mixed lymphocyte culture.Fas antigen exprexsion on the surface of T lymphocytes freshly isolated and activated was detected dynamically by using flow-cytometry(FCM).The influence of the Fas system activated or not on the rate of lymphocytic apoptosis after mixed culture was investigated by using DNA electrophoresis and TdT-mediated dUTP nick end labeling(TUNEL).Results The Fas expression on the surface of activated T lymphocytes was increased and the rate of apoptosis of the grow with addition of anti-Fas monoclonal antibody was higher than that of the group without addition of antibody(P<0.05).Conclusion It was possible to dispose of the lymphocytes activated by specific antigen in vitro by anti-Fas monoclonal antibody.
