Study on T cell tolerance induced by the combination of cyclosporin A and B7-1 monoclonal antibody
- VernacularTitle:环孢素A与抗B7-1单克隆抗体联用诱导T淋巴细胞耐受的研究
- Author:
Zhusen FAN
;
Baoli MA
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Antibodies,monoclonal;
Irmnune tolerance
- From:
Chinese Journal of Organ Transplantation
2000;21(3):161-163
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To study the induction and tolerance properties of antigen-specific T cell anergy.Methods APC:T:B cell reactive system was established in vitro.T cell proliferation was analyzed by using 3H-TdR incorporation in the blockade of B7/CD28 with B7-1 mAb and CsA.B cell antibody production was assayed by using ELISA ResultsAntigen-specific T cell anergy can be successfuily induced by the combination of CsA and B7-lmAb in vitro.Anergic T cell can not produce IL-2.but can secrete IL4.Anergic T cells can inhibit MLR.B cell proliferation and specific antibody production.Exogenous IL-2 can prevent T celt anergic induction,but can not reverse the state of tolerance.Conclusions Antigenspecific T cell anergy could be induced by CsA-combined with B7-1 mAb,which would provide theoretical evidence for prevention of transplantation rejection.
