Effect of human papilloma virus16 E6 shRNA mediated by lentivirus on infection ratio and invasive ability of cervical cancer cell
- VernacularTitle:慢病毒介导人乳头瘤病毒16型E6基因短发夹状干扰RNA对宫颈癌细胞感染效率和侵袭能力的影响
- Author:
Yao BAI
;
Jianxin GUO
;
Hui CHEN
;
Xiuhui ZHENG
;
Yaohua YAN
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
Human papilloma vires;
Lentivirus;
RNA interfering
- From:
Clinical Medicine of China
2008;24(5):440-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of lentivirus mediated short hairpin RNA(shRNA)target to human papilloma virus(HPV)16 E6 on invasive ability of cervical cancer Caski cells.Methods Lentivirus was produced after shRNA target to human papilloma virus(HPV)16 E6 and to nonsense was cloned to lentivirus work vector.Infection ratio was assessed by assay of EGFP positive cells of Caski.Total mRNA of E6 was determined by RT-PCR after Caski cells were infected by lentivirus.The change of E6 protein expression was analyzed by Western blot.The invasive ability of Caski cells was assayed employing Transwell.Results The optimal MOI (Multiplicity of infection)of lentivirus to Caski was 2.5.Total mRNA and protein of E6 were decreased (by 70%and 63%)in interfering group compared with control group.The invasive ability of Caski cells also reduced after infected by lentivirus.Conclusion shRNA mediated by lentivirus can inhibit expression of HPV16 E6 and invasive ability of cervical cancer cells.
