Preservation of isolated rabbit hearts after normothermia storage with stroma-free bovine hemoglobin solution
- VernacularTitle:牛血红蛋白用于兔离体心脏延时保存的研究
- Author:
Yunfan KANG
;
Zhenjie CAI
;
Jun HU
- Publication Type:Journal Article
- Keywords:
Stroma-free bovine hemoglobin;
Preservation solution;
Heart
- From:
Chinese Journal of Organ Transplantation
2000;21(2):95-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effects of stroma-free bovine hemoglobin(SFBHB)on preservation of the donor heart.Methods 24 isolated perfused rabbit hearts were equally divided into two groups.In control group,the cardiac arrest were induced with St Thomas Ⅱ solution and repeated every 30 min.the myocardial temperature remained at 40℃.In experimental group,the isolated rabbit hearts were perfused continuously with St Thomas Ⅱ solution containing stroma-free bovine hemoglobin and the myocardial temperature remained at 37℃.The preservation lasted 6 h.Results There were significant differences between the two groups in the follows:(1)The mean values of the left ventricular developed pressure(LVDP)and coronary flow rate(CFR)were higher in experimental group(P＜0.001);(2)The ATP level and myocardial oxygen uptake were significantly decreased in control group(P＜0.001);(3)The mean values of released creatine phosphate kinase(CPK),lactec dehydrogenase(LDH)and myocardial contents of malondialdehyde(MDA)were higher in control group(P＜0.01);(4)More water was present in the myocardial in control group(P＜0.01);(5)The myocardial ultrastructural injuries were greater in control group.Conclusion The stroma-free bovine hemoglobin could offer great protective effects on preservation of the isolated rabbit hearts.
