Microarray analysis of altered gene expression profile of hippocampus after traumatic brain injury in rats
- VernacularTitle:脑损伤大鼠海马差异表达基因的筛选
- Author:
Junfeng FENG
;
Kuiming ZHANG
;
Xian FU
;
Guoyi GAO
;
Yinghui BAO
;
Yumin LIANG
;
Yaohua PAN
;
Jiyao JIANG
- Publication Type:Journal Article
- Keywords:
Brain injuries;
Genomics;
Rats;
Hippocampus;
Gene expression chips
- From:
Chinese Journal of Trauma
2008;24(5):360-366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the altered gene expression profile of hippocampus after traumatic brain injury(TBI)in rats. Methods Rats(n=3)in experimental group underwent moderate fluid-percussion(F-P)brain injury and the hippoeampus sample in the injured hemisphere was removed and conserved in liquid nitrogen three hours later.The rats(n=5)of the control group underwent the same procedure except for injury.Mfymetrix rat genome 230 2.0 array was used to detect the gene expression profile of hippocampus in two groups and find the altered gene expression profile. Results A total of 159 genes in the experimental group changed significantly(≥2 folds)compared with the control group,of which 136 genes were up-regulated and 23 genes down-regulated. Conclusions The significant gene expression changes of hippocampus,especially a large mount of up-regulated genes,are detected after moderate TBI in rats,suggesting that the secondary injury following TBI is a procedure involving multiple factors.
