Study on the expression of free Ca2+ and AngiotensinⅡ1 type receptor in vascular smooth muscle cells of hypertensive coronary artery diseases
- VernacularTitle:高血压合并冠心病患者平滑肌细胞内Ca2+水平和血管紧张素Ⅱ1型受体表达的观察研究
- Author:
Ya LIU
;
Jielin LIU
;
Jie WEN
;
Limin ZHAO
;
Zuoguang WANG
;
Shaojun WEN
- Publication Type:Journal Article
- Keywords:
Hypertension;
Coronary artery heart disease;
AngiotensinⅡ1 receptors;
Ca2+;
Cell culture techniques;
Polymerase chain reaction
- From:
Clinical Medicine of China
2008;24(6):529-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of free Ca2+ and Angiotensin Ⅱ 1 type receptor(AT1 R)in vascular smooth muscle cells(VSMCs)of patients with hypertensive or normotensive coronary artery diseases(CAD).Methods During the coronary artery bypass graft operation,the surplus saphenous vein of patients who admitted to our Cardiac Surgery Department was collected and cultured.All patients were divided into hypertensive or normotensive group.Free Ca2+ in the cultured human VSMCs was determined by confocal laser scanning microscope(CLSM)after different kinds of AngiotensinⅡ being added,respectively.Total RNA was extracted from cultured VSMCs.Then RT-PCR was conducted for the observation of the expression of AT1R in both groups.Results Ca2+in human VSMCs rapidly increased when stimulated by Angtensin Ⅱ in two groups.After stimulated by Angiotensin Ⅱ,both free Ca2+ level and the expression of AT1R in VSMCs of hypertensive patients were higher than those of the normotensive patients(P＜0.05).Conclusion There are certain changes of free calcium in the cultured human vascular smooth muscle cells,when stimulated by Angiotensin Ⅱ.There are also difierences in AT1R expression between hypertensive CAD patients and normotensive CAD patients.
