Effects of ping-gan qianyang therapy on adrenal gland proteome in migraine rat model with hyperactivity of Hver-yang
- VernacularTitle:平肝潜阳法对偏头痛肝阳上亢型大鼠模型肾上腺组织蛋白质组的影响
- Author:
Jianjun HU
;
Zeqi CHEN
;
Guangwei ZHONG
;
Wei LI
;
Yaohui YIN
- Publication Type:Journal Article
- Keywords:
Migraine;
Proteomics;
Adrenal glands;
Drug therapy(TCD)
- From:
Journal of Chinese Physician
2008;10(5):590-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecule mechanisms of ping-gan qianyang therapy on migraine rat model with the hyperactivity of liver-yang.Methods An animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation and administering orally with Fuzi decoction.The total proteins of adrenal#and in the rats were separated by immobilized PH gradient-based two-dimensional gel electrophoresis, and the 2-DE images Was analyzed by PDQuest 7.0 software.Matrix-Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectrometry(MALDI-TOF-MS)and SWISS-PORT and MSDB database were used to identify differential proteins.Result The well-resolution and reproducible 2-DE patterns of rat adrenal glands in normal group,model group and therapy group were obtained.A total of 8 differentially expressed proteins were identified.Conclusion Ping-gan qianyang therapy may effect on migraine by regulating the expression of proteins related to energy metabolism and ubiquitin.
