Protective effects of cardiotrophin-1 gene transfection mediated by adnovirns on traumatic brain injuries
- VernacularTitle:心肌营养素-1基因转染对大鼠脑损伤的保护作用
- Author:
Yunhua YANG
;
Weihong LIAO
;
Hongyun LI
;
Yamin WU
;
Zhengfeng ZHANG
- Publication Type:Journal Article
- Keywords:
Brain injuries;
Cardiotrophin-1;
Gene therapy;
Apoptosis
- From:
Chinese Journal of Trauma
2008;24(5):355-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe biological effect of cardiotrophin-1(Adv-CT1)gene transfection mediated by adnovims on traumatic brain iniuries(TBI)in-vivo and discuss the role and mechanism of Adv-CT1 on TBI. Metheds A rat TBI model was established bv Allen method.After Adv-CT1 was transfefred into the iniured brain by adnovims,the effect of CT-1 on apoptosis and survival of neurons after TBI was determined by means of Nissl staining,TUNEL and flow cytometry apoptosis assay. Resuits Apoptotic cells were increased but the survived cells decreased in the injured cortical brain and hippocampus from 12 hours to 14 days after TBI in the control group.As compared with control group,Adv-CT1 treatment reversed this situation to some degrees. Conclusion CT-1 has neuropmtective effect on neurons after TBI by reducing apoptosis of neurons.
