Mutation of X region nucleotide sequence of hepatitis B virus DNA in patients with HBV reinfection after Hver transplantation
- VernacularTitle:肝移植术后HBV再感染患者HBV X区基因变异研究
- Author:
Zhongyang SHEN
;
Hongli SONG
;
Hong ZHENG
;
Jian WANG
;
Weiping ZHENG
;
Zhenglu WANG
;
Fan ZHANG
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Hepatitis B virus;
Nucleotides;
Sequence analysis;
Reinfection
- From:
Chinese Journal of General Surgery
2008;23(5):368-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the mutation of HBV X region nucleotide sequence in patients with HBV reinfection after liver transplantation. Methods In this study 320 patients received liver transplantation due to HBV-related end stage hver diseases between June 2002 and Dec 2003.Postoperatively polymerase chsin reaction was used to aInplify their serum HBV DNA fragments for direct sequence analysis.Patients that were followed-up for 1.5～3 years were enlisted for analysis.Results All the 11 reinfection recipients showed nucleotide mutations in X region mng4ng from 5 to 39 sites after transplantation.An A to T mutation at nt1762 and G to A mutation at nt1764 were found in 6 cases.The mutations at nucleotide fnt)1636～1741 were found in all 11 cases.Condusions The results indicated that mutations of nt 1762 and nt 1764 are very common and immunosuppressants cannot change the mutations in patients with HBV reinfection after liver transplantation.
