Effect of hyaluronic acid on mRNA expression of matrix metalloproteinases-3,9,13 and tissue in-hibitor of metalloproteinases-1 stimulated by interleukin-113 in chondrocytes from arthritis model in vitro
- VernacularTitle:透明质酸对白细胞介素-1 β诱导软骨细胞基质金属蛋白酶及其抑制因子mRNA表达的影响
- Author:
Panghu ZHOU
;
Shiqing LIU
- Publication Type:Journal Article
- Keywords:
Hyaluronic acid;
Interleukin-1;
Matrix metalloproteinases;
Chondrocytes
- From:
Chinese Journal of Rheumatology
2008;12(6):403-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Hyaluronic acid(HA) on the expression of matrix metalloproteinases(MMPs)-3,9,13 and tissue inhibitor of MMPs(TIMP)-1 mRNA stimulated by interleukin (IL)-1β in chondrocytes from arthritis model in vitro.Methods Different levels of Sodium Hyaluronate(10,20,40μg/ml)were added to culture medium of rat chondrocytes.The ehondrocytes were isolated from rat osteoarthritis model.One hour later,10 ng/ml IL-1β were added to each sample and co-cultured for 24 hours.The expressions of MMP-3,9,13 and TIMP-1 mRNA were detected bv RT-PCR.The concentration of nitric oxide (NO) in the supernatants were measured by Griess reaction.Results HA could down-regulate MMP-3,9,13 mRNA expression but had no effect on TIMP-1 mRNA expression.Moreover,it also had a dose-dependent down regulate effect on nitric oxide synthesis.Conclusion This study demonstrates that HA can down-regulate the TIMP-1/MMPs expression by inhibiting NO production.
