Expression of TβRⅡRH mRNA in PBMCs of patients with lupus nephritis
- VernacularTitle:转化生长因子βⅡ型受体在狼疮肾炎患者外周血单个核细胞表达的意义
- Author:
Chen ZHAO
;
Yongzhe LI
;
Dinghua LIU
;
Chaojun HU
;
Dawei TONG
;
Shulan ZHANG
;
Yang ZHAO
- Publication Type:Journal Article
- Keywords:
Lupus nephritis;
Transforming growth factor beta receptor;
Peripheral blood mononu-clear cells;
Reverse transcription-polymerase chain reaction
- From:
Chinese Journal of Rheumatology
2008;12(6):395-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of TGF-β receptor typeⅡ(TβRⅡ)mRNA with lupus nephritis (LN) and disease activity by testing its expression levelin peripheral blood mononuclear cells (PBMCs).Methotis Forty-four patients with LN were included in this study.They were all had active LN.Twepty-eight LN patients were taking glueocorticoids and/or immunosuppressive agents and sixteen had never taken steroids or immunosuppressive agents.The expression levels of T13R H mRNA were semi-quantitativelydetermined by reverse transcription-polymerase chain reaction(RT-PCR).Resuits The expression levels of TβRⅡ mRNA in PBMCs from LN patients(1.7±1.0)were lower than those of non-lupus nephritis(4.0±3.1) and healthy subiects(4.1±2.5),(P<0.01).The difference of the expression levels between patients who took and had never taken glucocorticoids and/or immunosuppressive drugs was significantly statistically(P<0.05).The expression levels of TβRⅡ mRNA in PBMCs of patients with LN were correlated significantly with the systemic lupus erythematosus disease activity index (SLEDAI)scores(r-0.309.P<0.05),titers of anti-dsDNA antibody(r=-0.401,P<0.01)and serum complement C3 level(r=0.621,P<0.01).Conclusion This study suggests that TβRⅡ may be involved in the development of LN,and the TβRⅡ mRNA expression levels in PBMCs from patients with SLE are significantly correlated with LN activity.Glucocortieoids or immunosuppressive drugs can increase the expression levels of TβRⅡ mRNA and ameliorate renal damage.
