Effect of X-irradiation on the protein expression of P57kip2 and TGF-β1 in lung cancer cell stain A549
- VernacularTitle:X射线对肺癌细胞株A549的P57kip2和TGF-β1蛋白表达的影响
- Author:
Huawei ZOU
;
Yonggang TAN
;
Heying ZHANG
- Publication Type:Journal Article
- Keywords:
X-rays;
Lung cancer cell stain;
p57kip2;
TGF-β1
- From:
Chinese Journal of Radiological Medicine and Protection
2008;28(3):249-251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect of X-irradiation on the proteins expression of p57kip2 and TGF-β1 in lung cancer cell stain A549 and its clinical significance.Methods Lung cancer cell stain A549 was cultivated and cell,protein was extracted at 6,12,24,36 and 48 hours after X-irradiation by differenl doses(2,4, 8 and 12 Gy).The expression of p57kip2 and TGF-β1 proteins were examined by Western blot.Results The expression of p57kip2 in lung cancer cell stain A549 was very low before X-irradiation.and increased significantly after irradiation with difierent doses and reached the peak level at 12 hours after irradiation(P<0.05).TGF-β1 reached its peak 1evel at 6 hours after irradiation(P<0.05).Conclusions X-irradiation can up-regulate the expression of p57kip2 and TGF-β1 proteins which increased with certain doses.p57kip2 and TGF-β1 could be usedto predict the damage degree of cancer cells by X-ray.
