Proteomic analysis of lung protein expression in rats inhaled radon
- VernacularTitle:吸入氡致大鼠肺蛋白质组表达变化的分析
- Author:
Naiyu XU
;
Suping ZHANG
;
Jihua NIE
;
Jiaosong DING
;
Jian TONG
- Publication Type:Journal Article
- Keywords:
Radon;
Lung;
Proteome
- From:
Chinese Journal of Radiological Medicine and Protection
2008;28(3):225-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the changes in protein profile of rats'lung inhaled radon using two- dimensional electrophoresis(2-DE)and MS.Methods Male Wistar rats were exposed to radon with the cumulative dose up to 100,200 and 400 working level months(WLM).The total proteins of rats'lung were extracted and isolated by 2-DE.The different protein expressions were analyzed with ImageMaster 2D Platinum software and obviously altered proteins were digested by trypsin and identified by MALDI-TOF-MS.Resuits Comparison of the 2-DE images between the control and radon-exposed groups indicated 14 up-regulated and 9 down-regulated protein spots,15 of which were identified by MALDI-TOF or MALDI-TOF/TOF MS.Conclusions The 2-DE image of radon groups'lung has changed,and the lung damage effect of radon is related to some proteins.
