Engraft of bone marrow multipotent adult progenitor cells into the wound skin in vivo
- VernacularTitle:大鼠骨髓多能成体祖细胞转化为皮肤组织细胞的在体研究
- Author:
Kaihong JI
;
Jun XIONG
;
Kaimeng HU
;
Lixing FAN
;
Houqi LIU
- Publication Type:Journal Article
- Keywords:
Adult stem cells;
Multipotent stem cells;
Skin;
Stem cell transplantation;
Rats
- From:
Chinese Journal of Trauma
2008;24(6):407-410
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the differentiation of bone marrow multipotent adult progenitor cells(MAPCs)into skin tissue cells of rats in vivo. Methods Magnetic activated cell sorting (MACS)was used to remove MAPCs from bone marrow of male rats through negative screening.Tail vein injection with combined with MAPCs Was done in C57BL/6 mice with skin wound and nude mice with immunodeficieney.Immunohistochemical staining was used to examine the expression of MHCI antigen in the healed skin of donor SD rats. Results Positive MHCI cells were found in the epidermal fundus and Some hair follicle-like structures of the healed skin of C57BL/6 mice.Hair follicle-like structure appeared in the healed skin of nude mice group,in which positive MHCI cells were found in the basal epidermal and some hair follicle-like structures. Conclusions During skin damage,MAPCs can migrate to the injured skin area and skin adnexa hair foilicle area,transform into epidermal cells and hence participate in the healing of the wound skin.
