Relationship between the expressions of lectin-like oxidized low-density lipoprotein receptor-1 and apoptosis associated genes in placenta tissues and the pathogenesis of preeclampsia
- VernacularTitle:胎盘组织中血凝素样氧化低密度脂蛋白受体1和凋亡相关基因的表达与子痫前期发病的关系
- Author:
Tao MENG
;
Jun LI
;
Haiying CHEN
;
Tao SHANG
- Publication Type:Journal Article
- Keywords:
Pre-eclampsia;
Placenta;
Scavenger receptors,class E;
Caspases;
Proto.oncogene proteins c-bcl-2: Apoptosis
- From:
Chinese Journal of Obstetrics and Gynecology
2008;43(6):405-409
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expressions of lectin-like oxidized low-density lipoprotein receptor-1(LOX-1)and apoptosis related genes caspase-3,Bax and bcl-2 in placenta,and their associations with the pathogenesis of preeclampsia.Methods Thirty preeclampsia patients(preeclampsia group),hospitalized in Department of Obstetrics,Shengiing Hospital of China Medical University from June 2005 to December 2006,were selected as the subject group,and 40 normal pregnant women as control group.The expressions of LOX-1 and apoptosis related genes caspase-3.Bax and bel-2 in different gestational weeks'placenta tissues were examined using immunohistochemistry.RT-PCR and western-blot.Results (1)Immunohistochemical detection in preeclampsia group:at 20 w+1-24 w,24 w+1-28 w,28 w+1-32 w.32w+1-36 w+1-36 w+1-40 w,respectively,the results of LOX-1 were 20.1±1.8,25.6±1.3,32.8±1.6,34.3 ±1.5,39.9±1.2;in the control group they respectively were 11.2±0.6,18.5±1.6,26.1±1.8,28.3 4-1.6,32.3±1.6;the difierence was significant(P<0.05).In preeclampsia group,the results of easpase-3 were 12.3±0.9,16.3±0.9,24.4 4-0.8,28.3±0.5,36.3±1.1.and in the control group they respectively were 8.5±1.0,12.3±1.1,17.4±1.2,20.4±stronger than those in normal pregnant women at different gestational weeks(P<0.05).However,the expression tendency of bcl-2 mRNA and proten was converse to the expression tendency of Bax.Conclusion The expressions of LOX-1,caspase-3,and Bax are upregulated in placentas of preeclampsia patients,while bcl-2 iS downregulated,both of which are associated with the pathogenesis of preeclampsia.
