A clinical and genetic analysis of a pedigree with two 46,XY patients suffering from 17α-hydroxylase deficiency
- VernacularTitle:两例46,XY同患17α-羟化酶缺陷症家系的临床和分子遗传学研究
- Author:
Jun LIANG
;
Jie QIAO
;
Xia CHEN
;
Qingqiang WU
;
Hao HENG
;
Tong ZHANG
;
Jiajun ZHAO
;
Huaidong SONG
- Publication Type:Journal Article
- Keywords:
Adrenal hyperplasia,congenital;
Mutation;
17α-hydroxylase deficiency
- From:
Chinese Journal of Internal Medicine
2008;47(6):482-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular defects of CYPl7A1 gene in a pedigree with two 46,XY patients suffering from 17α-hydroxylase deficiency (17-OHD) and explore the steroid biosynthetic difference in carriers of 17-OHD before and after adrenocorticotrophic hormone (ACTH) test.Methods Clinical data and hormone profiles were collected from the members of the pedigree.CYPl7A1 genotyping was performed in the patients and family members with PCR-direct sequencing.A short ACTH test was evaluated in some cases.Results The CYP17 genes of the patients were proved to hold a homozygous mutation with a base deletion and a base transversion (TAC/AA) in exon 6,which produced a missense mutation of Tyr→ Lvs at codon 329 and changed the open reading frame following this codon.The hormone response of the carriers after ACTH stimulation was abnormal between the patients and normal controls.Conclusion 17-OHD in this family was caused by CYP17A1 mutation (TAC329AA):some hormonal response to ACTH stimulation Was abnormal in carriers.
