Effects of HIF-1α on human gastric cancer cell's apoptosis in CO2 pneumoperitoneum environment
- VernacularTitle:缺氧诱导因子1α对CO2气腹环境下人胃癌细胞凋亡的影响
- Author:
Yingxue HAO
;
Hua ZHONG
;
Dongzhu ZENG
;
Yan SHI
;
Yun RAO
;
Lixin ZHOU
;
Peiwu YU
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Pneumoperitoneum;
artificial;
Apoptosis;
HIF-1α
- From:
Chinese Journal of General Surgery
2008;23(6):460-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of human gastric cancer cell's apoptosis in simulated CO2 pneumoperitoneum environment by HIF-1α. Methods We used a closed box to simulate CO2 pneumoperitoneum environment, a standard surgical insufflator maintained pressure of the box at 0,5,10 and 15 mm Hg respectively. MKN-45 cell's HIF-1α mRNA and protein expression were observed before and after silencing cell's HIF-1α by real time RT-PCR and Western blot. Cell's bcl-2/bax was studied by immunohistochemistry before and after silencing HIF-1α. Cell's apoptosis ratio was measured using Annexin V-FITC/PI double labelled staining. Results In 15 mm Hg group, MKN-45 cell's HIF-1α mRNA and protein expression were significantly higher than control group (P<0.01 ), while the difference was not significant among 0,5,10 mm Hg group and control group (P>0.05). In 15 mm Hg CO2 pressure, cell's bcl-2/bax was obviously lower than in control group (P<0.05) and apoptosis ratio was more than control group(P<0.01). When HIF-1α was silenced, cell's bel-2/bax and apoptosis ratio weren't significantly different between 15 mm Hg group and control group (P>0.05 ). Conclusions MKN-45 cell's apoptosis did not experience any alterations under 0,5,10 mm Hg.15 mm Hg CO2 pneumoperitoneum environment enhanced cell's apoptosis probably by way of HIF-1α.
