Study on the interaction between hepatitis B virus X protein and cytochrome c oxidase Ⅲ
- VernacularTitle:乙型肝炎病毒X蛋白与细胞色素C氧化酶Ⅲ的特异性结合
- Author:
Dan LI
;
Jian DING
;
Na LIN
;
Zhixin CHEN
;
Fenglin CHEN
;
Xiaozhong WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Viral proteins;
Cytochrome c oxidase Ⅲ;
Coimmunoprecipitation
- From:
Chinese Journal of Infectious Diseases
2008;26(6):341-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the interaction between hepatitis B virus(HBV) X protein and cytochrome C oxidase Ⅲ (COXⅢ).Methods Mating experiment and in vitro binding assays were utilized tO verify the interaction between HBV X protein and COX Ⅲ in vivo and in vitro respectively in yeast cells.Coimmunoprecipitation was employed to demonstrated the binding between these tWOproteins in mammalian cells.Results In mating experiment,the resulting diploids containing both pAS2-1-X and pACT2-COX Ⅲ plasmids turned blue in the β-gal assay,the result of Western blot displayed a positive reactive strap which indicated X protein could interact directly with COX Ⅲ in vitro binding assays.Interaction between these two proteins in mammalian cells was further confirmed by coimmunoprecipitation,as a protein strap about 17 000 was detected in Western blot.Conclusions It is speculated the specific interaction may exist between HBV X protein and COX Ⅲ in both prokaryotie and mammalian cells.X protein may impair the mitochondrial respiration chain and energy metabolism through its association with COX Ⅲ.
