The inhibitory effect of angiotensin Ⅱ type 1 receptor blocker combined with radiation on the proliferation and invasion ablility of human nasopharyngeal carcinoma cells
- VernacularTitle:血管紧张素Ⅱ受体阻滞剂联合放射对鼻咽癌细胞增殖和侵袭能力的影响
- Author:
Qiong WANG
;
Wei ZHAO
;
Guiling LI
;
Sheng ZHANG
;
Gang WU
- Publication Type:Journal Article
- Keywords:
Cell line,nasopharyngeal neoplasms;
Radiosensitization reagent;
Apoptosis;
Invasion
- From:
Chinese Journal of Radiation Oncology
2008;17(4):282-285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of valsartan,an angiotensinⅡtype 1 receptor (AT1 R)blocker,on radiosensitivity,invasive potential and proliferation activity of nasopharyngeal carcinoma cells(CNE-2)in vitro. Methods Radiosensitization of valsartan on CNE-2 cells in vitro was investigated by colony forming assay.Effect of ATl R blocker combined with radiation on invasive potential of CNE-2 cells was evaluated using 24-well Matrigel invasion chambers(Transwell).Apoptosis-inducing effect of valsartan combined with radiation on apoptosis of CNE-2 was identified by flowcytometry(FCM). Resuits When valsartan was given at 10-9.10-8 and 10-7 mol/L combined with radiation,sensitivity enhancement ratios (SER)were 1.10,1.20 and 1.36.and the invasive inhibition rates were 8.11%,16.49%and 16.77%,respectively.The SER of valsartan on CNE-2 distinctly increased when the exposure time was increased.After 24 h exposure to 10-8 mol/L valsartan combined witIl radiation.the apoptosis rate was 1.89%±0.09%,which was higher than 1.62%±0.06%in radiation alone group(t=4.79.P＜0.05). Conclusions AT1 R blocker valsartan combined with radiation can significantly inhibit the proliferation activity of nasophar,cngeal carcinoma cells in vitro in a dose- and time-dependent manner.Valsartan combined with radiation can potently inhibit the invasive potential of CNE-2.which may be involved in the mechanism of valsartan treatment in vivo.
