Effect of cefoprazone/sulbactam and dexamethasone on serunl IL-8,IL-10 in SD rats with severe pneumonia
- VernacularTitle:头孢哌酮/舒巴坦和地塞米松对细菌性重症肺炎大鼠血清IL-8,IL-10的影响
- Author:
Yijie ZHANG
;
Na CHEN
;
Jin MA
;
Jichang HAN
;
Yanqiu HU
- Publication Type:Journal Article
- Keywords:
Cefoperazone/AD;
Sulbactam/AD;
Dexamethasone/AD;
Pneumonia,bactrial;
Interleukin-8;
Interleukin-10
- From:
Journal of Chinese Physician
2008;10(6):742-745
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of cefoprazone/sulbactam(CPZ/Sul)and dexamethasone(DXM) on serum IL-8,IL-10 in SD rats with severe pneumonia due to Klebsiella pneumoniae.Methods A rat model of severe pneumonia was induced by intratra- cheal instillation of Klebsiella pneumoniae with a dose of 3.6×108CFU(0.3ml).60 male SD rats were random divided into control group,model group,cefoprazone/sulbactam(CF-L/Sul)group and cefoprazone/sulbactam plus dexamethasone(DXM+CPZ/Sul)group.15 rats in each group were then divided into three subgroups and 5rats in each subgroup were examined at the 6th,8th and 10th day,respectively.Arterial gas analysis.total WBC and PMN cell counts in BALF were examined.ELISA method was used to detect the level of IL-8 and IL-10 in serum.RESULTSIn model group,PaO2 were progressively decreased while total WBC and PMN cell counts in BALF were markedly increased.In CPZ/Sul group,all parameters were alleviated,but there were no significant difference of serum IL-8 and IL-10 between model group and CPZ/Sul group.In DXM+CPZ/SUl group,all parameters were significantly alleviated including serum IL-8 and IL-10.Conclu sions Severe pneumonia of rat due to Klebsiella pneumoniae was characterized by significant elevation of serum IL-8 and IL-10,which suggested the imbalance of inflammatory/anti-inflammatory mediators during the course of severe pneumonia.DXM codd markedly decrease the production of inflammatory mediators and slightly decrease the production of anti-inflammatory mediators,which could restore the balance and contribute to the relief of severe pneumonia.
