Effect of low molecular weight substance extracted from chick embryo on mitochondrial DNA deletion in aging mice induced by D-galactose
- VernacularTitle:鸡胚低分子提取物对D-半乳糖拟衰老小鼠线粒体DNA缺失突变的影响
- Author:
Shu CHEN
;
Yunshao HE
;
Gang CHENG
;
Jinsong GAO
- Publication Type:Journal Article
- Keywords:
Galactose;
Aging;
DNA,mitochondrial;
Mutation
- From:
Chinese Journal of Geriatrics
2008;27(7):544-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of low molecular weight substance extracted from chick embryo on mitochondrial DNA(mtDNA)deletion in senile mice induced bv D-galactose.Methods Senile mice induced by D-galactose were treated with low molecular weight substance extracted from chick embryo.The deleted fragment of mtDNA was examined by using polymerase chain reaction technique and agarose gel electrophoresis.A relative quantitation of band densities was performed by using densitometry scanning techniques.The deletion was identified by using direct sequencing analysis. Results The 4239 bD mtDNA deletion were present in liver,cerebral cortex and hippocampus tissues in all of the mice.and it was significantly higher in senile model mice than in control mice(all P＜0.01).Low molecular weight substance extracted from chick embryo reduced the mtDNA deletion in various tissues in senile model mice(all P＜0.05).Deletion was more abundant in liver than in cerebral cortex and hippoeampus. Conclusions The 4239 bp mtDNA deletion is a common deletion,and low molecular weight substance extracted from chick embryo could decrease the incidence of 4239 bD mtDNA deletion.
