Targeted killing of malignant melanoma cells by aclarubicin liposome conjugated with vascular endothelial growth factor
- VernacularTitle:血管内皮生长因子-阿柔比星脂质体靶向杀伤恶性黑素瘤细胞的实验观察
- Author:
Hongxiang CHEN
;
Qiang TONG
;
Yue QIAN
;
Yan WU
;
Aiping FENG
;
Zhihong WU
;
Xiaofeng YAN
;
Yating TU
- Publication Type:Journal Article
- Keywords:
Melanoma;
Receptors,vascular endothelial growth factor;
Aclarubicin;
Liposomes
- From:
Chinese Journal of Dermatology
2008;41(7):429-432
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the targeted killing of malignant melanoma cells by aclarubicin liposomes conjugated with vascular endothelial growth factor(ADM-VEGF-SSL)in vitro.Metheds To detect the binding abilitv of liposomes to malignant melanoma(MM)cells,the human malignant melanoma cell line A375 was cultured in the presence of ADM-VEGF-3H-SSL or ADM-3H-SSL for 2 days followed by the detection of radioactivity of these cells.Then.A375 cells were cultured with various concentrations(0.01,0.1,1,10,100 mol/L)of ADM-VEGF-SSL,ADM-SSL or free ADM for 48 hours in the 48-hour cytotoxity test,or for 0.5 hour followed by another 48-hour culture in drug-free medium in the 0.5-hour cytotoxity test.After that,MTT assay was used to detect the survival rate of these cells.Results ADM-VEGF-SSL could specifically bind to and kill A375 cells.The binding rate of ADM-VEGF-SSL was 2.15 folds as high as that of ADM-SSL.The survival rate of A375 cells after being treated with ADM-VEGF-SSL for 48 hour was similar to that with flee ADM(P＞0.05).but lower than that with ADM-SSL(P＜0.05),while the survival rate of melanocytes treated with ADM-VEGF-SSL was higher than that with free ADM or ADM-SSL(both P＜0.05).As shown by the 0.5-hour cytotoxity test.shortening the treatment course did not attenuate the effect of ADM-VEGF-SSL on A375 cells.Conclusions ADM-VEGF-SSL can specifically recognize A375 cells.efficiently deliver adriamycin into tumor cells,markedly inhibit the proliferation of A375 cells,and eventually,a targeted kill of these cells is realized.
