Applications and advances of flow fluorescence microbeads array
- VernacularTitle:流式荧光微球技术的应用现状及进展
- Author:
Jinli HAN
;
Xixing KANG
- Publication Type:Journal Article
- Keywords:
Fluorescence microbeads;
Flow cytometry;
High thoughput
- From:
International Journal of Biomedical Engineering
2008;31(3):172-175
- CountryChina
- Language:Chinese
-
Abstract:
Flow fluorescence microbeads array is a novel high throughput technique based on the combina tion of use of flow cytometry and use of fluorescence encoded microbeads as the solid phase for conventional im munoassay,affinity assay and DNA hybridization assay,.which offers a new approach to multiplexed assays for ap plications of large-scale screening.By utilizing appropriate fluorescence encoded micron-sized polymer particles, flow fluorescence microbeads array can be created to enable highly muhiplexed analysis of complex samples.Indi vidual sets of microbeads can be modified with reactive components such as antigens,antibodies or oligonu eleotides,then mixed with each other to form a multiplexed assay set.Flow fluorescence microbeads array com bines the specificity of fluorescence-encoded microbeads and the high sensitivity of flow cytometry,bears signifi cant potential as a general analysis platform for both research and clinical applications.Recent applications and advances are reviewed in this article.
