Immunogenicity of a novel vaccine with VLP as vector for Wilm's tumor
- VernacularTitle:以病毒样颗粒为载体的小肾母细胞瘤新型疫苗的研制
- Author:
Zheng WANG
;
Yueqiang DUAN
;
Deyan LUO
;
Xiliang WANG
;
Zhoulu LIU
- Publication Type:Journal Article
- Keywords:
Nephroblstoma;
Viral fusion proteins;
Vaccines;
DNA
- From:
Chinese Journal of General Surgery
2008;23(6):457-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a vaccine for Wilm's tumor in order to lay a foundation for effective treatment and substitute for the radiotherapy and chemotherapy of this tumor. Methods DNA fragments encoding the WT1 antigens (including, HLA-A * 2402 and HLA-A * 2402x), and DNA fragments encoding the couple antigens HLA-A * 2402-HBc and HLA-A * 2402x-HBc were cloned and inserted into the eukaryotic expression vector pcDNA3.1(+) in sense orientation and baculovirus expression pFactbac respectively, then transfected into COS-7 cells and Sf 9 cell respectively. The expression of the target proteins in the cells were identified by CPE, SDS-PAGE and electron microscopy. Virus-like particles were observed under electron microscopy. Results The fusion gene of HLA-A * 2402 and HLA-A * 2402x and HBc express virus-like particle protein. Conclusions This preliminary result shows a hopeful future in developing an effective immunotherapy to Wilm's tumor.
