Protective effect of cobalt protoporphyrin-induced the strong expression of heme oxygenase-1 on islet xenotransplant
- VernacularTitle:钴原卟啉诱导血红素氧化酶-1表达上调对异种移植胰岛的保护作用
- Author:
Zhengyun ZHANG
;
Xi CHEN
;
Chang SU
;
Weiqiong GU
;
Hongwei LI
;
Guangwen ZHOU
- Publication Type:Journal Article
- Keywords:
Islets of langerhans transplantation;
Transplantation,heterologous;
Graft rejeetion;
Heme oxygenase
- From:
Chinese Journal of Organ Transplantation
2008;29(6):343-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the dose-effect relationship between cobalt protoporphyrin (CoPP) and heme oxygenase-1 (HO-1) expression in islets and to investigate the protective effect of strongly expression of HO-1 in islet xenotransplantation. Methods Donor islets isolated and purified from SD rats were randomly divided into 5 groups and incubated with different doses of CoPP for 24 h.Group A: 0 mmol/L; Group B: 5 mmol/L; Group C: 25 mmol/L; Group D: 50 mmol/L; Group E:75 mmol/L. The expression of HO-1 mRNA and protein in islets was detected by RT-PCR and Western blot respectively. Glucose of low and high concentrations was added to islets in vitro to test insulin-releasing function. The optimal dose of CoPP which could induce the strongest HO-1 expression was chosen according to the results. Recipients were randomly divided into 2 groups. Control group received untreated xeno-islets, and the experimental group received islets incubated with optimal CoPP close in vitro. Glycemia and rejection were observed after transplantation daily. Results The expression of HO-1 mRNA and protein in xeno-islets of group D was significantly higher than in other groups (P＜0.05). After stimulation of glucose, the insulin concentration in group D was significantly higher than in other groups (P＜0.05). The optimal dose of CoPP which could induce the strongest HO-1 expression was 50 mmol/L. The time for normoglyeemia in experimental group was (14.63±1.19) days, significantly longer than that in control group (9.88±2.17)days (P＜0.01). Conclusion The strongest expression of HO-1 induced by CoPP in vitro promotes the glucose-stimulated insulin secretion of islets and prolonged the survival of xeno-islet grafts by protecting them from rejection.
