Relationship of neutrophil apoptosis and caspase-3 activity with multiple organ dysfunction syn-drome after multiple injury
- VernacularTitle:中性粒细胞凋亡及caspase-3活性与多发伤患者MODS的关系
- Author:
Ling LIN
;
Tao ZHU
;
Konghan PAN
;
Xiujun CAI
;
Qi WANG
- Publication Type:Journal Article
- Keywords:
Multiple injury;
Caspase-3;
Neutrophils;
Apoptesis
- From:
Chinese Journal of Trauma
2008;24(7):548-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate apoptosis of polymorphonuclear neutrophil and activity of caspase-3 in the peripheral blood and discuss their correlation with development of multiple organ dysfunc- tion syndrome (MODS) after multiple injury. Methods A total of 55 patients with multiple injury were included in the prospective study, and divided into two groups, ie, MODS group (multiple injury patients who developed MODS,) and non-MODS group (multiple injury patients who were free from MODS). The activity of caspase-3 was detected by flow cytometry and the serum levels of IL-6 and IL-10 were detected by ELISA. Then, we evaluated whether the neutrophil apoptesis was correlated with the ser- um levels of IL-6 and IL-10 in patients with MODS. Results Compared with non-MODS group, neu- trophil apoptesis was significantly reduced and activated caspase-3 decreased significantly in MODS group (P <0.05). In MODS group, serum IL-6 was increased significantly while serum IL-10 was decreased significantly compared with non-MODS group. The apoptosis of neutrophil in multiple injury patients with MODS had a negative correlation with IL-6 levels but a positive correlation with IL-10 levels. Conclu- sions The delayed apoptosis of neutrophil due to decreased activated caspase-3 may play partial roles in the development of MODS after multiple injury. IL-6 and IL-10 may contribute to the apoptotic changes.
