Effects of lipoxygenase inhibitor NDGA on expression of 5-LOX and its apoptosis related genes in HepG2 cell line
- VernacularTitle:NDGA对HepG2细胞5-LOX及其凋亡相关基因表达的影响
- Author:
Lixiang LI
;
Qiru XIONG
;
Xiaoping GENG
;
Lixin ZHU
;
Yeben QIAN
;
Hongzhu YU
;
Yechuan XU
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
oncogenes;
genes;
lipoxygenase inhibitors
- From:
International Journal of Surgery
2008;35(7):448-451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lipoxygenase inhibitor NDGA on expression of 5-LOX and its apoptosis related genes in HepG2 cell line.Methods The expression of 5-LOX and apoptosis related genes hTERT,bcl-2 and bax mRNA was determined by reverse transcriptasepolymerase chain reaction (RT-PCR).Results After 25,50,100,200 μmol/L NDGA treatment for24,48 h,the expression of 5-LOX of HepG2 cell decreased,but the expression of bax was up-regulated and the expressions of bcl-2 and hTERT mRNA were down-regulated,(P＜0.05 compared with the control group).The decrease in the expression of 5-LOX,hTERT and bcl-2 in HepG2 cell was negtively correlated with the dose duration of action of NDGA.Conclusion In vitro,5-LOX is expressed highly in HepG2 cell.Overexpression of 5-LOX may be related to the progression of hepatocellular carcinoma,NDGA can significantly decrease the expression of 5-LOX,up-regulate of bax and downregulation of bcl-2 and telomerse.Lipoxygenase might be a novel therapeutic target for the hepatocellular carinoma.
