Reverse mode of Na+/Ca2+ exchange inhibitor, KB-r7943 attenuates tubular epithelial cell apoptosis inducde by contrast media
- VernacularTitle:反向模式钠钙离子交换体抑制剂降低造影剂诱导的肾小管上皮细胞凋亡
- Author:
Dingping YANG
;
Ruhan JIA
;
Guohua DING
;
Dingwei YANG
;
Xiaoling XIONG
- Publication Type:Journal Article
- Keywords:
Contrast Media;
Cell Apoptosis;
Na+/Ca2+ exchanger inhibitor
- From:
Chinese Journal of Emergency Medicine
2008;17(7):713-716
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the renoprotective effects of KB-R7943 on renal injury induced bycontrast media. Method Tubular epithelial cells were trested with varions cencentration of KB-B7943 (10-5,10-6 mol/L) for 12 hours before contrast media was used. After cells we, re incubated with contrast media (CM)(110 mgI/L) for 1 hour, cells injury was assessed by using LDH, and cell morphologic changes and cells apopto-sis were evaluated with inverted microscope and flow cytometry, respoelively. Intracellular Ca2+ and reactive oxy-gen species (ROS) were analyzed by using confocal microscope. The expression of Na+/Ca2+exchanger mRNAwas evaluated by RT-PCR. Mannitol with same osmolarity (20% mannitol, 770 mOsm/L) of CM was used as con-trol. One-way analysis of variance and q-test were used for comparison between groups. The simple linear correla-tion was employed to analyze the correlation. P < 0.05 was considered significant. Results Contrast media sig-nificantly induced tubular cells damage significantly and apoptosis at 1 hour after incubation, meanwhile, intracel-lular Ca2+ and ROS were inoreased progressively in CM group. KB-R7943 significantly attenuated cells damage andapoptosis in dose-dependent in association with decreased intracellular Ca2+ and ROS. Expression of Na+/Ca2+exchanger mRNA was not changed. Conclusions KB-R7943 has renoprotective effects on the contrast-media-in-duced renal tubular cyotoxicity
