Suppression effects of adenovirus-mediated PTEN gene transfer on growth and Invasion of human EL22 cells in vitro
- VernacularTitle:腺病毒介导PTEN基因抑制肝癌体外生长和侵袭力的研究
- Author:
Qing YANG
;
Huawen SUN
;
Zuojiong GONG
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Genes,tumor suppressor;
Adenoviridae
- From:
Journal of Chinese Physician
2008;10(7):889-891
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of adenovirus-thediated PTEN gene transfer on growth and invasion of H22 cells in vitro. Methods H22 cells were tnmsfected with Ad-PTEN and Ad-PTENG-129R it vitro, The mRNA expressions of PTEN were detected with RT-PCR. Cell viability was determined by MTT assay. Invasion of H22 in vitro was observed using Boyden chamber. Results Gene and protein expressions of PTEN were shown to be up-regulated when H22 was transfected with Ad-PTEN. MTT assay showed that the group transfected with Ad-PTEN had much lower cell viability than other groups (P<0.05) . The number of 1422 invasion cells in the group of Ad-PTEN was (15.64±3.27) %, which was lower than the number in other groups (P<0.05). Conclusion These results imply that the number of H22 invasion cells was significantly decreased after infected with Ad-PTEN in vitro.
