The effects of the proliferation of adipose-derived adult stromal cells stimulated by granulocyte colony-stimulating factor
- VernacularTitle:粒细胞集落刺激因子对脂肪基质细胞增殖的影响
- Author:
Youqing LIN
;
Silang ZHOU
;
Zeyin ZHUANG
- Publication Type:Journal Article
- Keywords:
Granulocyte colony stimulating factor;
Adipose-derived adult stromal cells;
Cell proliferation;
Cellcycle
- From:
Chinese Journal of Primary Medicine and Pharmacy
2008;15(7):1110-1112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of granulocyte colony-stimulating factor on proliferation of adipose-derived adult stromal cells(ADASc) in rats. Methods Sixteen SD rats were randomly divided into G-CSF group( n = 8) and control group( n = 8). The rats were subjected to subcutaneous injections of G-CSF at a dose of The ADASc were separated and cultured. Then: ( 1 ) The surface antigens of the ADASc were analyzed by flow cy- tometry. (2)The growth information of the ADASc were observed in vitro. (3)The generation cycle of the ADASc were investigated with the flow cytometry. Results ( 1 ) Flow cytometic detection of ADASe surface marks showed CD44+CD105-CD31-CD45-(2)The doubling generation time, the maximum proliferation multiple and the cell cycle distri- bution of ADASc in G-CSF group had significant difference from that of the control group. Conclusion The discrep- ancy adherence was a practical method to culture the ADASc;G-CSF treatment could promote ADASc re-entering into cell cycle.
