Influence of hypothermia on the expression of calpain Ⅱ and microtubule associated protein 2 mRNA in the lateral fluid percussion injury rats
- VernacularTitle:亚低温对液压脑损伤大鼠钙蛋白酶Ⅱ及微管结合蛋白2 mRNA表达的影响
- Author:
Xian FU
;
Guoyi GAO
;
Jun PU
;
Junfeng FENG
;
Yingming ZHANG
;
Zhongtang FENG
;
Jiyao JIANG
;
Wei XU
- Publication Type:Journal Article
- Keywords:
Traumatic brain injuy;
Calpain Ⅱ;
MAP2;
Hypothermia
- From:
Clinical Medicine of China
2008;24(8):737-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the early expression of ca]pain Ⅱ and microtubule associated protein 2 (MAP2) mRNA in the hippocampus of the lateral fluid percussion injury rats. Methods 18 Male Sprague-Dawley rats were randomly divided into 3 groups. The changes of Calpaln Ⅱ and MAP2 mRNA in hippocampus 3 h after injury were detected by real-time PCR. Results Compared to the control group (n = 6), the expression for Ca]pain Ⅱ mRNA increased obviously(P <0.01)in the lateral fluid percussion injury group(n=6) ,the expression for MAP2 mRNA degraded obviously(P <0.01). Compared with the lateral fluid percussion injury group(n =6) ,the expression for calpuin Ⅱ mRNA in the mild hypothermia group degraded obviously (n = 6), the expression for MAP2 mRNA increased obviously(P <0.01). Conclusion Mild hypothermia may act as neuroprotection by inhibiting the expression of Ca]pain Ⅱ and easing the degradation of cytoskeleton.
