Expression of signal transduction molecule STAT-1 in patients with chronic hepatitis B after interferon-alpha therapy
- VernacularTitle:STAT-1分子在干扰素治疗慢性乙型肝炎不同应答者肝组织中的表达
- Author:
Luwen WANG
;
Zuojiong GONG
- Publication Type:Journal Article
- Keywords:
Janus kinase;
Signal transduction;
Activating transcription factor;
Chronic hepatitis B;
Interferon
- From:
Chinese Journal of Clinical Infectious Diseases
2008;1(2):77-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of signal transduction molecule STAT-1 in patients with chronic hepatitis B (CHB) who received interferon-alpha (IFNα) treatment with different responses. Methods A total of 13 CHB patients received IFNα treatment, among who 5 demonstrated complete viral response ( Group A) and 8 showed no response ( Group B). All patients underwent liver biopsy before and after the therapy. STAT-1 mRNA of liver tissue was semi-quantified by RT-PCR and levels of STAT-1 protein were detected by Western-blot. Results Absorbance values (A) of STAT-1 mRNA in group A and B were (1. 18±0.06) and (0.21±0.04) respectively (t =35.27, P<0. 01). The levels of STAT-1 protein of liver tissue in group A were also significantly higher than those in group B. Conclusion IFNα resistance may be closely related to the down-regulation of STAT-1.
