Effects of Smad7 on AGEs-induced NRK52E cell transdifferentiation
- VernacularTitle:Smad 7对AGEs介导肾小管上皮细胞转分化的影响
- Author:
Liao SUN
- Publication Type:Journal Article
- Keywords:
DNA-binding proteins;
Glyeoslation end products,advanced;
Kidney tubules;
Endothelial cells;
Cell differentiation
- From:
Journal of Chinese Physician
2008;10(7):865-868
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the effect of smad7 on AGEs-induced NRK52E cell tranedifferentiation. Methods NRK52E cells were transferred with Smad7 gene and the cell lines of doxcycllne (Dox) -regulated Smad7 expression were selected for the study. The NRK52E cells were random divided into two groups, Dox - group, the NRK52E cells were cultured with medium only containing AGEs, Dox +group, the NRK52E cells were cultured with medium containing AGEs and Dox. Nuclear translation of p-Smad2/3 were examined with immunocytochemistry. The expression of SmadT, α-SMA, E-cadherin protein were detected by western blot. Results Expression of Smad7 protein in the Dox + group was increased higher than that in Dos- group( t = 3.05, P<0.01). High expression of Smad7 caused a marked inhibition of p-Smad2/3 transnuclear location at 30min and 24h(t=4. 5,t= 3.2, P<0.01). Overexpression of Smad7 dramatically de- creased protein expression of α-SMA, while evidently increased protein of E-cadherin in the NRK52E cells stimulated by AGEs(t=3.47, t=3.25, P<0.01). Conclusion Overexpression of Smad7 can inhibit AGEs - induced transdifferentiation in NRK52E cells.
