Effects of VEGF on proliferation and morphology of hBMSCs
- VernacularTitle:血管内皮生长因子对人骨髓基质细胞增殖的影响及其形态学观察
- Author:
Bin CHEN
;
Yanbin SONG
;
Guoxian PEI
- Publication Type:Journal Article
- Keywords:
Bone marrow cells;
Vascular endothelial growth factor (VEGF);
Cell proliferation
- From:
Chinese Journal of Orthopaedic Trauma
2008;10(7):659-661
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influences of VEGF transfection on the proliferation and ultrastructure of hBMSCs. Methods Three groups were enrolled in the experiment: group of Ad-VEGF transfection, group of empty Ad transfection, and the control group. Cells were observed continually by inverted phase contrast microscope, and stained by HE. Proliferation of cells was tested by MTT and by flow cytometry analysis. Results Histological observation and observation through inverted phase contrast microscope showed that the cells were of the similar morphology in the 3 groups. As time elapsed, the amount of cells increased, but still no obvious differences were found in optical density (OD) value of hBMSCs.Groups B, C, A had a similar percentage of DNA G1 period and a similar proliferation index (PrI) ( P >0. 05). Conclusion Transfeetion of VEGF has no obvious influence on the prohferation and morphologyof hBMSCs in vitro.
